LabVine Learning Courses - Fluoride Determination by Ion-Selective Electrode

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Fluoride Determination by Ion-Selective Electrode

Measurement of Fluoride with ISE Electrode: instrument, theory, operation, care, etc.


About Fluoride Determination by Ion-Selective Electrode

Fluoride is present in water and many food products. Small concentrations are beneficial to health, but large concentrations are toxic. Measurement is performed conveniently with inexpensive equipment. The equipment is introduced, the principles explained, and operation, calibration, care, maintenance and troubleshooting covered in this course.

Developed by:
Osborne Analytical
2 hours
Learning Mode:
Certificate of completion


  • Introduction
  • Equipment
  • Theory
  • Electrodes
  • Sample collection and preparation
  • Calibration and measurement
  • Care and maintenance
  • Troubleshooting
  • Field Measurements



John Osborne

John has worked in the field of analytical chemistry for over 45 years and has gained experience in a wide variety of analytical techniques and instrumentation in several industries and applications. He has always been interested in understanding the technology well and utilizing it at its optimum in order to produce reliable and accurate chemical data. To this end he is committed to enable others to not only use the technology correctly but also understand what it does, how it works and how to maintain it.

Chapter 1: Fluoride Determination by Ion-Selective Electrode

Part 1

30 minutes

The topic is introduced, and the measurement process described and explained. Theory of measurement is explained by way of the Nernst equation. We then deal with the principle of the electrodes and their construction.

Part 2

20 minutes

In Part 2, the attention is turned to water sample collection and preparation, operation then calibration and measurement. We include notes on interferences and matrix effects which can affect results.

Part 3

1 hour

Part 3 is concerned with care and maintenance of the meter and electrodes, and troubleshooting (identifying and correcting problems should they occur). We conclude with some tips on field measurement and determination of very low concentrations.