LabVine Learning Courses - Ultraviolet and Visible Spectrophotometry

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Ultraviolet and Visible Spectrophotometry

This course looks at UV-Vis spectrophotometry techniques, instruments, operations, maintenance and troubleshooting.


About Ultraviolet and Visible Spectrophotometry

This course introduces the subject of Spectroscopy in general with the basic principles of behaviour of electromagnetic waves and how they interact with matter. We discuss atomic and molecular transitions, transmittance and absorbance, operation and calibration, methodologies, maintenance and troubleshooting.

Developed by:
Osborne Analytical
3 hours
Learning Mode:
Certificate of completion


  • Electromagnetic Radiation
  • Introduction to Spectroscopy
  • Theory of Measurement
  • UV-Visible Spectrophotometers
  • Operation
  • Calibration and Measurement
  • UV-Visible measurement methods
  • Maintenance and Troubleshooting



John Osborne

John has worked in the field of analytical chemistry for over 45 years and has gained experience in a wide variety of analytical techniques and instrumentation in several industries and applications. He has always been interested in understanding the technology well and utilizing it at its optimum in order to produce reliable and accurate chemical data. To this end he is committed to enable others to not only use the technology correctly but also understand what it does, how it works and how to maintain it.

Chapter 1: Ultraviolet and Visible Spectrophotometry

Part 1

40 minutes

In the first section, the basics of electromagnetic radiation are explained followed by the theory of UV-Visible spectrometry including Atomic and molecular absorption, beer’s Law and the principles of the measurement.

Part 2

40 minutes

Instrument components are described and instrument layout, measurement cells, the method of wavelength separation and some instrument design.

Part 3

30 minutes

Some safety information is shared, start-up and shut-down procedures with examples from an available instrument. Guidance on setting up the instrument to run a method, and calibration are covered, including information on calibration curves and quality control.

Part 4

1 hour

This section is devoted to care and maintenance. The information is generic but with some examples on the available instrument. Some problems that can occur separate from the instrument are also described, i.e. to do with sample or standard preparation.